ABSTRACT
There is a growing awareness of a need for developing novel methods of contraceptive technology which should not only be effective in providing protection against conception but also take into consideration the reproductive health issues confronting men and women. This paper considers the process of embryo implantation as one such potential target. The hormonal basis of embryo implantation in primates has been discussed to indicate that progesterone, and not estrogen, from ovarian source is the primary determinant of embryo-endometrial maturation and their synchronization for implantation. Thus, low dose administration of the anti-progesterone, mifepristone, during early luteal phase has been shown to be an effective anti-implantation approach to for fertility control. Furthermore, the dissociation of endometrial-hormonal synchrony at the time of blastocyst implantation following the post-ovulatory mifepristone administration has been shown to be the physiological basis of its anti-implantation effect with undisturbed circulatory hormone profiles and ovarian functions. Further studies are required to appreciate the full potential and to mollify the limitations of this approach.
Subject(s)
Animals , Contraceptives, Oral, Synthetic/administration & dosage , Embryo Implantation/drug effects , Embryonic Development/drug effects , Embryonic and Fetal Development/physiology , Endometrium/drug effects , Estrogens/physiology , Estrus Synchronization/drug effects , Female , Fertility/drug effects , Hormone Antagonists/administration & dosage , Humans , Mifepristone/administration & dosage , Ovary/metabolism , Pregnancy , Primates , Progesterone/antagonists & inhibitorsABSTRACT
Air proved to be a superior gaseous medium to pure oxygen used for oxygen uptake study in the Warburg apparatus Recording of volume change (vessel sensitivity method) was the only correct method of recording the oxygen uptake. It is concluded that the volume change method should be followed for recording oxygen uptake by Warburg apparatus and pure oxygen should not be used as a gaseous medium.
Subject(s)
Air , Animals , Blood Gas Analysis/instrumentation , Chickens/blood , Erythrocytes/metabolism , Manometry , Oxygen/blood , Oxygen Consumption , Partial PressureABSTRACT
Taking oxygen and air as gaseous media oxygen uptake study was carried out recording delta V and corresponding delta P. From these data the quantity of oxygen consumed was calculated. It was concluded that delta V x vessel sensitivity x 2 = delta P x vessel constant. Recording of volume change in oxygen uptake study has been advocated.